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Crystal structure of NucB, a biofilm-degrading endonuclease.

ABSTRACT: Bacterial biofilms are a complex architecture of cells that grow on moist interfaces, and are held together by a molecular glue of extracellular proteins, sugars and nucleic acids. Biofilms are particularly problematic in human healthcare as they can coat medical implants and are thus a potential source of disease. The enzymatic dispersal of biofilms is increasingly being developed as a new strategy to treat this problem. Here, we have characterized NucB, a biofilm-dispersing nuclease from a marine strain of Bacillus licheniformis, and present its crystal structure together with the biochemistry and a mutational analysis required to confirm its active site. Taken together, these data support the categorization of NucB into a unique subfamily of the ??? metal-dependent non-specific endonucleases. Understanding the structure and function of NucB will facilitate its future development into an anti-biofilm therapeutic agent.

SUBMITTER: Basle A

PROVIDER: S-EPMC5758888 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Crystal structure of NucB, a biofilm-degrading endonuclease.

Baslé Arnaud A Hewitt Lorraine L Koh Alan A Lamb Heather K HK Thompson Paul P Burgess J Grant JG Hall Michael J MJ Hawkins Alastair R AR Murray Heath H Lewis Richard J RJ

Nucleic acids research 20180101 1

Bacterial biofilms are a complex architecture of cells that grow on moist interfaces, and are held together by a molecular glue of extracellular proteins, sugars and nucleic acids. Biofilms are particularly problematic in human healthcare as they can coat medical implants and are thus a potential source of disease. The enzymatic dispersal of biofilms is increasingly being developed as a new strategy to treat this problem. Here, we have characterized NucB, a biofilm-dispersing nuclease from a mar ...[more]

PMID: 29165717

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