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Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering.

ABSTRACT: Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.

SUBMITTER: Wei L

PROVIDER: S-EPMC4040164 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering.

Wei Lu L Hu Fanghao F Shen Yihui Y Chen Zhixing Z Yu Yong Y Lin Chih-Chun CC Wang Meng C MC Min Wei W

Nature methods 20140302 4

Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors. ...[more]

PMID: 24584195

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