Ontology highlight
ABSTRACT:
SUBMITTER: Zorzini V
PROVIDER: S-EPMC4041440 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Zorzini Valentina V Buts Lieven L Sleutel Mike M Garcia-Pino Abel A Talavera Ariel A Haesaerts Sarah S De Greve Henri H Cheung Ambrose A van Nuland Nico A J NA Loris Remy R
Nucleic acids research 20140419 10
The Staphylococcus aureus genome contains three toxin-antitoxin modules, including one mazEF module, SamazEF. Using an on-column separation protocol we are able to obtain large amounts of wild-type SaMazF toxin. The protein is well-folded and highly resistant against thermal unfolding but aggregates at elevated temperatures. Crystallographic and nuclear magnetic resonance (NMR) solution studies show a well-defined dimer. Differences in structure and dynamics between the X-ray and NMR structural ...[more]