Loop-mediated isothermal amplification test for detection of Neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea.
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ABSTRACT:
SUBMITTER: Edwards T
PROVIDER: S-EPMC4042760 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Publications
Loop-mediated isothermal amplification test for detection of Neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea.
Edwards Thomas T Burke Patricia A PA Smalley Helen B HB Gillies Liz L Hobbs Glyn G
Journal of clinical microbiology 20140312 6
A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤ 1.8 M, compared with a PCR assay that was functional at concentrations of <100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform. ...[more]
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