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A Loop-Mediated Isothermal Amplification Assay Targeting Neisseria gonorrhoeae penA-60.001.

ABSTRACT: Ceftriaxone (CRO) is widely used as the first-line treatment for gonococcal infections. However, CRO-resistant Neisseria gonorrhoeae strains carrying mosaic penA-60.001 have emerged recently and disseminated worldwide. To meet the urgent need to detect these strains, we report here a loop-mediated isothermal amplification (LAMP) assay system that targets N. gonorrhoeae penA-60.001. This assay system can differentiate N. gonorrhoeae strains carrying mosaic penA-60.001 from strains carrying other penA alleles.

SUBMITTER: Shimuta K

PROVIDER: S-EPMC7187580 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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A Loop-Mediated Isothermal Amplification Assay Targeting Neisseria gonorrhoeae penA-60.001.

Shimuta Ken K Nakayama Shu-Ichi SI Takahashi Hideyuki H Ohnishi Makoto M

Antimicrobial agents and chemotherapy 20191220 1

Ceftriaxone (CRO) is widely used as the first-line treatment for gonococcal infections. However, CRO-resistant Neisseria gonorrhoeae strains carrying mosaic penA-60.001 have emerged recently and disseminated worldwide. To meet the urgent need to detect these strains, we report here a loop-mediated isothermal amplification (LAMP) assay system that targets N. gonorrhoeaepenA-60.001. This assay system can differentiate N. gonorrhoeae strains carrying mosaic pen ...[more]

PMID: 31658968

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Correction: Clinical Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Neisseria meningitidis in Cerebrospinal Fluid.

Project description:[This corrects the article DOI: 10.1371/journal.pone.0122922.].

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