Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones.

ABSTRACT: CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6-yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P2?, with unit-cell parameters a=70.0, b=104.8, c=73.5?Å, ?=114.4°.

SUBMITTER: Yang D 

PROVIDER: S-EPMC4051524 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature