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Crystallization and preliminary X-ray crystallographic studies of the ArsI C-As lyase from Thermomonospora curvata.

ABSTRACT: Arsenic is a ubiquitous and carcinogenic environmental element that enters the biosphere primarily from geochemical sources, but also through anthropogenic activities. Microorganisms play an important role in the arsenic biogeochemical cycle by biotransformation of inorganic arsenic into organic arsenicals and vice versa. ArsI is a microbial nonheme ferrous-dependent dioxygenase that transforms toxic methylarsonous acid to the less toxic inorganic arsenite by C-As bond cleavage. An ArsI ortholog from the thermophilic bacterium Thermomonospora curvata was expressed, purified and crystallized. The crystals diffracted to 1.46?Å resolution and belonged to space group P4?2?2 or its enantiomer P4?2?2, with unit-cell parameters a=b=42.2, c=118.5?Å.

SUBMITTER: Nadar SV

PROVIDER: S-EPMC4051532 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray crystallographic studies of the ArsI C-As lyase from Thermomonospora curvata.

Nadar S Venkadesh SV Yoshinaga Masafumi M Kandavelu Palani P Sankaran Banumathi B Rosen Barry P BP

Acta crystallographica. Section F, Structural biology communications 20140510 Pt 6

Arsenic is a ubiquitous and carcinogenic environmental element that enters the biosphere primarily from geochemical sources, but also through anthropogenic activities. Microorganisms play an important role in the arsenic biogeochemical cycle by biotransformation of inorganic arsenic into organic arsenicals and vice versa. ArsI is a microbial nonheme ferrous-dependent dioxygenase that transforms toxic methylarsonous acid to the less toxic inorganic arsenite by C-As bond cleavage. An ArsI ortholog ...[more]

PMID: 24915088

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