Ontology highlight
ABSTRACT:
SUBMITTER: Jain R
PROVIDER: S-EPMC4061903 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Jain Rinku R Vanamee Eva S ES Dzikovski Boris G BG Buku Angeliki A Johnson Robert E RE Prakash Louise L Prakash Satya S Aggarwal Aneel K AK
Journal of molecular biology 20131019 2
DNA polymerase ε (Polε) is a multi-subunit polymerase that contributes to genomic stability via its roles in leading strand replication and the repair of damaged DNA. Polε from Saccharomyces cerevisiae is composed of four subunits--Pol2, Dpb2, Dpb3, and Dpb4. Here, we report the presence of a [Fe-S] cluster directly within the active polymerase domain of Pol2 (residues 1-1187). We show that binding of the [Fe-S] cluster is mediated by cysteines in an insertion (Pol2(ins)) that is conserved in Po ...[more]