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A red-shifted fluorescent substrate for aldehyde dehydrogenase.

ABSTRACT: Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH(pos) cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH(pos) murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.

SUBMITTER: Minn I

PROVIDER: S-EPMC4063304 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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A red-shifted fluorescent substrate for aldehyde dehydrogenase.

Minn Il I Wang Haofan H Mease Ronnie C RC Byun Youngjoo Y Yang Xing X Wang Julia J Leach Steven D SD Pomper Martin G MG

Nature communications 20140423

Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH(pos) cells in the pre ...[more]

PMID: 24759454

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