A Fast-Response Red Shifted Fluorescent Probe for Detection of H2S in Living Cells.

ABSTRACT: Near-infrared (NIR) fluorescent probes are attractive tools for bioimaging applications because of their low auto-fluorescence interference, minimal damage to living samples, and deep tissue penetration. H2S is a gaseous signaling molecule that is involved in redox homeostasis and numerous biological processes in vivo. To this end, we have developed a new red shifted fluorescent probe 1 to detect physiological H2S in live cells. The probe 1 is based on a rhodamine derivative as the red shifted fluorophore and the thiolysis of 7-nitro 1,2,3-benzoxadiazole (NBD) amine as the H2S receptor. The probe 1 displays fast fluorescent enhancement at 660 nm (about 10-fold turn-ons, k2 = 29.8 M-1s-1) after reacting with H2S in buffer (pH 7.4), and the fluorescence quantum yield of the activated red shi

SUBMITTER: Ismail I 

PROVIDER: S-EPMC7036821 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature