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Rapid adaptive optical recovery of optimal resolution over large volumes.

ABSTRACT: Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.

SUBMITTER: Wang K

PROVIDER: S-EPMC4069208 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Rapid adaptive optical recovery of optimal resolution over large volumes.

Wang Kai K Milkie Daniel E DE Saxena Ankur A Engerer Peter P Misgeld Thomas T Bronner Marianne E ME Mumm Jeff J Betzig Eric E

Nature methods 20140413 6

Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafi ...[more]

PMID: 24727653

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