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Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes

ABSTRACT: An innovative imaging tool enables in vivo high-resolution visualization of deep brain structures and functions over large volume. Optical deep-brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep-brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.

SUBMITTER: Qin Z

PROVIDER: S-EPMC7527232 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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