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Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae.

ABSTRACT: The mechanism of transport through the Golgi complex is not completely understood, insofar as no single transport mechanism appears to account for all of the observations. Here, we compare the transport of soluble secretory proteins (albumin and ?1-antitrypsin) with that of supramolecular cargoes (e.g., procollagen) that are proposed to traverse the Golgi by compartment progression-maturation. We show that these soluble proteins traverse the Golgi much faster than procollagen while moving through the same stack. Moreover, we present kinetic and morphological observations that indicate that albumin transport occurs by diffusion via intercisternal continuities. These data provide evidence for a transport mechanism that applies to a major class of secretory proteins and indicate the co-existence of multiple intra-Golgi trafficking modes.

SUBMITTER: Beznoussenko GV

PROVIDER: S-EPMC4070021 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae.

Beznoussenko Galina V GV Parashuraman Seetharaman S Rizzo Riccardo R Polishchuk Roman R Martella Oliviano O Di Giandomenico Daniele D Fusella Aurora A Spaar Alexander A Sallese Michele M Capestrano Maria Grazia MG Pavelka Margit M Vos Matthijn R MR Rikers Yuri G M YG Helms Volkhard V Mironov Alexandre A AA Luini Alberto A

eLife 20140527

The mechanism of transport through the Golgi complex is not completely understood, insofar as no single transport mechanism appears to account for all of the observations. Here, we compare the transport of soluble secretory proteins (albumin and α1-antitrypsin) with that of supramolecular cargoes (e.g., procollagen) that are proposed to traverse the Golgi by compartment progression-maturation. We show that these soluble proteins traverse the Golgi much faster than procollagen while moving throug ...[more]

PMID: 24867214

Dataset Information

Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae.

Publications

Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae.

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