Single-cell western blotting.
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ABSTRACT:
SUBMITTER: Hughes AJ
PROVIDER: S-EPMC4077215 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Publications
Single-cell western blotting.
Hughes Alex J AJ Spelke Dawn P DP Xu Zhuchen Z Kang Chi-Chih CC Schaffer David V DV Herr Amy E AE
Nature methods 20140601 7
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h. A microscope slide supporting a 30-μm-thick photoactive polyacrylamide gel enables western blotting: settling of single cells into microwells, lysis in situ, gel electrophoresis, photoinitiated blotting to immobilize proteins and antibody probing. We applied this scWestern method to monitor single-cell differentiation of rat neural ...[more]
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