Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting.
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ABSTRACT:
SUBMITTER: Duncombe TA
PROVIDER: S-EPMC4708057 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Publications
Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting.
Duncombe Todd A TA Kang Chi-Chih CC Maity Santanu S Ward Toby M TM Pegram Mark D MD Murthy Niren N Herr Amy E AE
Advanced materials (Deerfield Beach, Fla.) 20151116 2
Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast bi ...[more]
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