Separation-encoded microparticles for single-cell western blotting.
Ontology highlight
ABSTRACT:
SUBMITTER: Gumuscu B
PROVIDER: S-EPMC7029799 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Publications
Separation-encoded microparticles for single-cell western blotting.
Lab on a chip 20191127 1
Direct measurement of proteins from single cells has been realized at the microscale using microfluidic channels, capillaries, and semi-enclosed microwell arrays. Although powerful, these formats are constrained, with the enclosed geometries proving cumbersome for multistage assays, including electrophoresis followed by immunoprobing. We introduce a hybrid microfluidic format that toggles between a planar microwell array and a suspension of microparticles. The planar array is stippled in a thin ...[more]
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