Ontology highlight
ABSTRACT:
SUBMITTER: Suzuki J
PROVIDER: S-EPMC4082642 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Suzuki Junji J Kanemaru Kazunori K Ishii Kuniaki K Ohkura Masamichi M Okubo Yohei Y Iino Masamitsu M
Nature communications 20140613
The endoplasmic reticulum (ER) and mitochondria accumulate Ca(2+) within their lumens to regulate numerous cell functions. However, determining the dynamics of intraorganellar Ca(2+) has proven to be difficult. Here we describe a family of genetically encoded Ca(2+) indicators, named calcium-measuring organelle-entrapped protein indicators (CEPIA), which can be utilized for intraorganellar Ca(2+) imaging. CEPIA, which emit green, red or blue/green fluorescence, are engineered to bind Ca(2+) at i ...[more]