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An expanded set of fluorogenic sulfatase activity probes.

ABSTRACT: Fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. Here, we describe two fluorogenic enzyme probes, 3-O-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. Both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. The fluorogenic probes described here are suitable for various assays and provide a starting point for creating new sulfatase probes with improved selectivity for mycobacterial sulfatases.

SUBMITTER: Smith EL

PROVIDER: S-EPMC4084507 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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An expanded set of fluorogenic sulfatase activity probes.

Smith Elizabeth L EL Bertozzi Carolyn R CR Beatty Kimberly E KE

Chembiochem : a European journal of chemical biology 20140424 8

Fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. Here, we describe two fluorogenic enzyme probes, 3-O-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. Both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. The fluorogenic ...[more]

PMID: 24764280

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