Ontology highlight
ABSTRACT:
SUBMITTER: Casasampere M
PROVIDER: S-EPMC6547634 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Journal of lipid research 20190329 6
New fluorogenic ceramidase substrates derived from the <i>N</i>-acyl modification of our previously reported probes (RBM14) are reported. While none of the new probes were superior to the known RBM14C12 as acid ceramidase substrates, the corresponding nervonic acid amide (RBM14C24:1) is an efficient and selective substrate for the recombinant human neutral ceramidase, both in cell lysates and in intact cells. A second generation of substrates, incorporating the natural 2-(<i>N</i>-acylamino)-1,3 ...[more]