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Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense.

ABSTRACT: A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a ?-glucan with a backbone of (1?4)- and (1?6)-Glcp, bearing terminal- and (1?3)-Glcp side-chains at O-3 position of (1?6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.

SUBMITTER: Han XQ

PROVIDER: S-EPMC4094382 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense.

Han Xiao-Qiang XQ Yue Gar-Lee GL Yue Rui-Qi RQ Dong Cai-Xia CX Chan Chung-Lap CL Ko Chun-Hay CH Cheung Wing-Shing WS Luo Ke-Wang KW Dai Hui H Wong Chun-Kwok CK Leung Ping-Chung PC Han Quan-Bin QB

PloS one 20140711 7

A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and ...[more]

PMID: 25014571

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