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Structural and functional studies indicate that Shigella VirA is not a protease and does not directly destabilize microtubules.

ABSTRACT: VirA, an essential virulence factor in Shigella disease pathogenesis, is involved in the uptake, motility, and cell-to-cell spread of Shigella organisms within the human host. These functions have been attributed to a VirA protease activity and a mechanism of microtubule destruction via tubulin degradation [Yoshida, S., et al. (2006) Science 314, 985-989]. We report functional and crystallographic data indicating a novel VirA structure that lacks these activities but highlights the homology to the EspG virulence factor of pathogenic Escherichia coli.

SUBMITTER: Germane KL

PROVIDER: S-EPMC4096815 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

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Structural and functional studies indicate that Shigella VirA is not a protease and does not directly destabilize microtubules.

Germane Katherine L KL Ohi Ryoma R Goldberg Marcia B MB Spiller Benjamin W BW

Biochemistry 20080903 39

VirA, an essential virulence factor in Shigella disease pathogenesis, is involved in the uptake, motility, and cell-to-cell spread of Shigella organisms within the human host. These functions have been attributed to a VirA protease activity and a mechanism of microtubule destruction via tubulin degradation [Yoshida, S., et al. (2006) Science 314, 985-989]. We report functional and crystallographic data indicating a novel VirA structure that lacks these activities but highlights the homology to t ...[more]

PMID: 18763811

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2019-06-04 | PXD012291 | Pride

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