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High-throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Twelve microsatellite markers were developed for Primula mistassinica, a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. • METHODS AND RESULTS:We used Roche/454 high-throughput technology to sequence microsatellite-enriched regions in the P. mistassinica genome. We developed 12 polymorphic microsatellite primer sets. These loci contained di-, tri-, and tetranucleotide repeats with two to nine alleles per locus when assessed in 23 individuals. • CONCLUSIONS:Understanding the historical movements of P. mistassinica will provide insight to the survival prospects of current Arctic plant populations, which face the pressures of global, anthropogenic climate change.

SUBMITTER: Matheny H

PROVIDER: S-EPMC4103446 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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High-throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae).

Matheny Hannah H Edwards Joan J Maroja Luana S LS

Applications in plant sciences 20130715 8

<h4>Unlabelled</h4><h4>Premise of the study</h4>Twelve microsatellite markers were developed for Primula mistassinica, a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. •<h4>Methods and results</h4>We used Roche/454 high-throughput technology to sequence microsatellite-enriched regions in the P. mistassinica genome. We developed 12 polymorphic ...[more]

PMID: 25202573

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