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Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distylous-homostylous species, using MiSeq sequencing.

ABSTRACT: Primula chungensis is a species with considerable floral and mating-system variation, including distylous (outcrossing), homostylous (selfing) and mixed populations that contain both outcrossing and selfing forms. We isolated 24 microsatellite markers from P. chungensis using Illumina MiSeq sequencing. Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet. All loci were polymorphic with the number of alleles per locus ranging from 2 to 4. The observed and expected heterozygosity ranged from 0 to 1 and 0.219 to 0.708, respectively. The microsatellite markers we have identified will serve as valuable tools for the investigation of the population genetic structure and phylogeography of P. chungensis and will inform models of the evolutionary history of mating systems in the species.

SUBMITTER: Zhou W

PROVIDER: S-EPMC6112135 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distylous-homostylous species, using MiSeq sequencing.

Zhou Wei W Li Haidong H Wu Zhikun Z Barrett Spencer C H SCH Li Dezhu D Wang Hong H

Plant diversity 20160401 2

Primula chungensis is a species with considerable floral and mating-system variation, including distylous (outcrossing), homostylous (selfing) and mixed populations that contain both outcrossing and selfing forms. We isolated 24 microsatellite markers from P. chungensis using Illumina MiSeq sequencing. Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet. All loci were polymorphic with the numbe ...[more]

PMID: 30159452

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