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Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • METHODS AND RESULTS:DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pairs, seven of which were polymorphic in P. boveana. Eleven of these primers successfully cross-amplified to related taxa. • CONCLUSIONS:The markers reported herein will be useful to characterize the genetic diversity of the endangered P. boveana and to evaluate its mating system, and have the potential to be useful for similar studies in close relatives.

SUBMITTER: Mansour H

PROVIDER: S-EPMC4105029 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae).

Mansour Hassan H Jiménez Ares A Keller Barbara B Nowak Michael D MD Conti Elena E

Applications in plant sciences 20130507 6

<h4>Unlabelled</h4><h4>Premise of the study</h4>We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. •<h4>Methods and results</h4>DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pairs, seven of which were polymorphic in P. boveana. Eleven of these primers successful ...[more]

PMID: 25202556

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