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Isolation and characterization of microsatellite loci in the invasive herb Solidago altissima (Asteraceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite markers were developed in the perennial herb Solidago altissima from populations within its introduced range in Japan to assess its population structure and to facilitate tracking of invasion expansion. • METHODS AND RESULTS:Using 454 pyrosequencing, 16 microsatellite primer sets were developed for S. altissima. The primer sets were tested on 70 individuals sampled from three populations in Japan. The primers amplified di- and trinucleotide repeats with five to 25 alleles per locus, and the expected heterozygosity ranged from 0.46 to 0.92. • CONCLUSIONS:These results indicate the utility of primers in S. altissima for future research on a wide range of applications, including tracking of invasion dynamics and investigating population genetics of the species.

SUBMITTER: Sakata Y

PROVIDER: S-EPMC4105288 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Isolation and characterization of microsatellite loci in the invasive herb Solidago altissima (Asteraceae).

Sakata Yuzu Y Kaneko Shingo S Hayano Azusa A Inoue-Murayama Miho M Ohgushi Takayuki T Isagi Yuji Y

Applications in plant sciences 20130322 4

<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite markers were developed in the perennial herb Solidago altissima from populations within its introduced range in Japan to assess its population structure and to facilitate tracking of invasion expansion. •<h4>Methods and results</h4>Using 454 pyrosequencing, 16 microsatellite primer sets were developed for S. altissima. The primer sets were tested on 70 individuals sampled from three populations in Japan. The primers amplified di- and ...[more]

PMID: 25202531

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