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Genus-wide microsatellite primers for the goldenrods (Solidago; Asteraceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus Solidago. • METHODS AND RESULTS:Illumina sequencing of a shotgun library from S. gigantea identified ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifiable, single-copy, and variable in a broad range of Solidago species. • CONCLUSIONS:The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate numerous inter- and intraspecific studies in the ca. 120 Solidago species.

SUBMITTER: Beck JB

PROVIDER: S-EPMC4103136 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Genus-wide microsatellite primers for the goldenrods (Solidago; Asteraceae).

Beck James B JB Semple John C JC Brull Justin M JM Lance Stacey L SL Phillips Mai M MM Hoot Sara B SB Meyer Gretchen A GA

Applications in plant sciences 20140404 4

<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus Solidago. •<h4>Methods and results</h4>Illumina sequencing of a shotgun library from S. gigantea identified ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifiable, single-copy, and variable in a broad range of Solidago species. •<h4>Conclusions</h4>The utilit ...[more]

PMID: 25202617

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