Ontology highlight
ABSTRACT:
SUBMITTER: Kellie JF
PROVIDER: S-EPMC4107347 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Kellie John F JF Higgs Richard E RE Ryder John W JW Major Anthony A Beach Thomas G TG Adler Charles H CH Merchant Kalpana K Knierman Michael D MD
Scientific reports 20140723
A robust top down proteomics method is presented for profiling alpha-synuclein species from autopsied human frontal cortex brain tissue from Parkinson's cases and controls. The method was used to test the hypothesis that pathology associated brain tissue will have a different profile of post-translationally modified alpha-synuclein than the control samples. Validation of the sample processing steps, mass spectrometry based measurements, and data processing steps were performed. The intact protei ...[more]