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Identification and Quantification of Proteoforms by Mass Spectrometry.

ABSTRACT: A proteoform is a defined form of a protein derived from a given gene with a specific amino acid sequence and localized post-translational modifications. In top-down proteomic analyses, proteoforms are identified and quantified through mass spectrometric analysis of intact proteins. Recent technological developments have enabled comprehensive proteoform analyses in complex samples, and an increasing number of laboratories are adopting top-down proteomic workflows. In this review, some recent advances are outlined and current challenges and future directions for the field are discussed.

SUBMITTER: Schaffer LV

PROVIDER: S-EPMC6602557 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Identification and Quantification of Proteoforms by Mass Spectrometry.

Schaffer Leah V LV Millikin Robert J RJ Miller Rachel M RM Anderson Lissa C LC Fellers Ryan T RT Ge Ying Y Kelleher Neil L NL LeDuc Richard D RD Liu Xiaowen X Payne Samuel H SH Sun Liangliang L Thomas Paul M PM Tucholski Trisha T Wang Zhe Z Wu Si S Wu Zhijie Z Yu Dahang D Shortreed Michael R MR Smith Lloyd M LM

Proteomics 20190501 10

A proteoform is a defined form of a protein derived from a given gene with a specific amino acid sequence and localized post-translational modifications. In top-down proteomic analyses, proteoforms are identified and quantified through mass spectrometric analysis of intact proteins. Recent technological developments have enabled comprehensive proteoform analyses in complex samples, and an increasing number of laboratories are adopting top-down proteomic workflows. In this review, some recent adv ...[more]

PMID: 31050378

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