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Screening proteins for NMR suitability.


ABSTRACT: NMR spectroscopy is a valuable tool in structural genomics. Identification of protein samples that are amenable to structure determination by NMR spectroscopy requires efficient screening. The preparation of multiple samples in parallel and screening by NMR is described. The method described is applicable to large structural genomics projects but can easily be scaled down for application to small structural biology projects. All the equipment used is commonly found in any NMR structural biology laboratory.

SUBMITTER: Yee AA 

PROVIDER: S-EPMC4113955 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Screening proteins for NMR suitability.

Yee Adelinda A AA   Semesi Anthony A   Garcia Maite M   Arrowsmith Cheryl H CH  

Methods in molecular biology (Clifton, N.J.) 20140101


NMR spectroscopy is a valuable tool in structural genomics. Identification of protein samples that are amenable to structure determination by NMR spectroscopy requires efficient screening. The preparation of multiple samples in parallel and screening by NMR is described. The method described is applicable to large structural genomics projects but can easily be scaled down for application to small structural biology projects. All the equipment used is commonly found in any NMR structural biology  ...[more]

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