Ontology highlight
ABSTRACT:
SUBMITTER: Peng Y
PROVIDER: S-EPMC4117494 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Peng Yu Y Yu Ge G Tian Shaoxiong S Li Hong H
PloS one 20140731 7
Box C/D ribonucleoprotein particles (RNPs) are 2'-O-methylation enzymes required for maturation of ribosomal and small nuclear RNA. Previous biochemical and structural studies of the box C/D RNPs were limited by the unavailability of purified intact RNPs. We developed a bacterial co-expression strategy based on the combined use of a multi-gene expression system and a tRNA-scaffold construct that allowed the expression and purification of homogeneous archaeal and human box C/D RNPs. While the co- ...[more]