Ontology highlight
ABSTRACT:
SUBMITTER: Fu L
PROVIDER: S-EPMC4119833 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Fu Li L Li Lingyun L Cai Chao C Li Guoyun G Zhang Fuming F Linhardt Robert J RJ
Analytical biochemistry 20140606
The thermal instability of the anticoagulant heparin is associated, in part, with the solvolytic loss of N-sulfo groups. This study describes a new method to assess the increased content of unsubstituted amino groups present in thermally stressed and autoclave-sterilized heparin formulations. N-Acetylation of heparin samples with acetic anhydride-d6 is followed by exhaustive heparinase treatment and disaccharide analysis by hydrophilic interaction chromatography mass spectrometry (HILIC-MS). The ...[more]