An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture.

ABSTRACT:

Background

In vitro blood-brain barrier (BBB) models can be useful for understanding leukocyte-endothelial interactions at this unique vascular-tissue interface. Desirable features of such a model include shear stress, non-transformed cells and co-cultures of brain microvascular endothelial cells with astrocytes. Recovery of transmigrated leukocytes for further analysis is also appealing.

New methods

We report an in vitro BBB model for leukocyte transmigration incorporating shear stress with co-culture of conditionally immortalized human endothelial cell line (hBMVEC) and human astrocyte cell line (hAST). Transmigrated leukocytes can be recovered for comparison with input and non-transmigrated cells.

Result

hBMVEC and hAST exhibited physiological and morphological BBB

SUBMITTER: Takeshita Y 

PROVIDER: S-EPMC4122330 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature