Ontology highlight
ABSTRACT:
SUBMITTER: Kim H
PROVIDER: S-EPMC4125384 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Kim Heesun H Ishidate Takao T Ghanta Krishna S KS Seth Meetu M Conte Darryl D Shirayama Masaki M Mello Craig C CC
Genetics 20140530 4
Genome editing based on CRISPR (clustered regularly interspaced short palindromic repeats)-associated nuclease (Cas9) has been successfully applied in dozens of diverse plant and animal species, including the nematode Caenorhabditis elegans. The rapid life cycle and easy access to the ovary by micro-injection make C. elegans an ideal organism both for applying CRISPR-Cas9 genome editing technology and for optimizing genome-editing protocols. Here we report efficient and straightforward CRISPR-Ca ...[more]