Ontology highlight
ABSTRACT:
SUBMITTER: Quick-Cleveland J
PROVIDER: S-EPMC4133150 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Quick-Cleveland Jen J Jacob Jose P JP Weitz Sara H SH Shoffner Grant G Senturia Rachel R Guo Feng F
Cell reports 20140606 6
Canonical primary microRNA transcripts (pri-miRNAs) are characterized by a ∼30 bp hairpin flanked by single-stranded regions. These pri-miRNAs are recognized and cleaved by the Microprocessor complex consisting of the Drosha nuclease and its obligate RNA-binding partner DGCR8. It is not well understood how the Microprocessor specifically recognizes pri-miRNA substrates. Here, we show that in addition to the well-known double-stranded RNA-binding domains, DGCR8 uses a dimeric heme-binding domain ...[more]