Ontology highlight
ABSTRACT:
SUBMITTER: Agrawal AA
PROVIDER: S-EPMC4786784 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Nature communications 20160308
How the essential pre-mRNA splicing factor U2AF(65) recognizes the polypyrimidine (Py) signals of the major class of 3' splice sites in human gene transcripts remains incompletely understood. We determined four structures of an extended U2AF(65)-RNA-binding domain bound to Py-tract oligonucleotides at resolutions between 2.0 and 1.5 Å. These structures together with RNA binding and splicing assays reveal unforeseen roles for U2AF(65) inter-domain residues in recognizing a contiguous, nine-nucleo ...[more]