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Spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors.

ABSTRACT: Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.

SUBMITTER: Ulissi ZW

PROVIDER: S-EPMC4134139 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors.

Ulissi Zachary W ZW Sen Fatih F Gong Xun X Sen Selda S Iverson Nicole N Boghossian Ardemis A AA Godoy Luiz C LC Wogan Gerald N GN Mukhopadhyay Debabrata D Strano Michael S MS

Nano letters 20140730 8

Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on average ...[more]

PMID: 25029087

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