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Two-color, two-photon uncaging of glutamate and GABA.

ABSTRACT: We developed a caged GABA (gamma-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs.

SUBMITTER: Kantevari S

PROVIDER: S-EPMC4135702 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Two-color, two-photon uncaging of glutamate and GABA.

Kantevari Srinivas S Matsuzaki Masanori M Kanemoto Yuya Y Kasai Haruo H Ellis-Davies Graham C R GC

Nature methods 20091227 2

We developed a caged GABA (gamma-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pa ...[more]

PMID: 20037590

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