Unknown

Dataset Information

0

Genetic variants of APOC3 promoter and HLA-B genes in an HIV infected cohort in northern South Africa: a pilot study.


ABSTRACT: Metabolic disorders and hypersensitivities affect tolerability and impact adherence to highly active antiretroviral therapy (HAART). The aim of this study was to determine the prevalence of C-482T/T-455C variants in the Apolipoprotein C3 (APOC3) promoter gene and Human leukocyte antigen (HLA)-B*57:01, known to impact lipid metabolic disorders and hypersensitivity respectively; and to correlate genotypes with gender, CD4+ cell count and viral load in an HIV infected cohort in northern South Africa. Frequencies of C-482 and T-455 polymorphisms in APOC3 were determined by restriction fragment length polymorphism analysis. Allele determination for HLA-B was performed with Assign SBT software in an HLA library. Analysis of APOC3 C-482 site revealed a prevalence of 196/199 (98.5%) for CC, 1/199 (0.5%) for CT and 2/199 (1.0%) for TT genotype (p = 0.000 with 1° of freedom; ?2 = 126.551). For the T-455 site, prevalences were: 69/199 (35%) for TT and 130/199 (65%) for the CC genotype (p = 0.000 with 1° of freedom; ?2 = 199). There was no association between gender and the presence of -482 (p = 1; ?2 = 0.00001) or -455 genotypes (p = 0.1628; ?2 = 1.9842). There was no significant difference in the increase in CD4+ cell count irrespective of genotypes. Significant increases in CD4+ cell count were observed in males and females considering the -455C genotype, but not in males for the -455T genotype. Viral load decreases were significant with the -455C and -482C genotypes irrespective of gender. HLA-B*57:01 was not identified in the study cohort. The apparently high prevalence of APOC3 T-455CC genotype needs confirmation with a larger samples size and triglyceride measurements to support screening of patients to pre-empt HAART associated lipid disorders.

SUBMITTER: Masebe T 

PROVIDER: S-EPMC4139789 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

altmetric image

Publications

Genetic variants of APOC3 promoter and HLA-B genes in an HIV infected cohort in northern South Africa: a pilot study.

Masebe Tracy T   Bessong Pascal Obong PO   Ndip Roland Ndip RN   Meyer Debra D  

International journal of molecular sciences 20140626 7


Metabolic disorders and hypersensitivities affect tolerability and impact adherence to highly active antiretroviral therapy (HAART). The aim of this study was to determine the prevalence of C-482T/T-455C variants in the Apolipoprotein C3 (APOC3) promoter gene and Human leukocyte antigen (HLA)-B*57:01, known to impact lipid metabolic disorders and hypersensitivity respectively; and to correlate genotypes with gender, CD4+ cell count and viral load in an HIV infected cohort in northern South Afric  ...[more]

Similar Datasets

| S-EPMC6339282 | biostudies-literature
| S-EPMC6467198 | biostudies-literature
| S-EPMC4465896 | biostudies-literature
| S-EPMC8237893 | biostudies-literature
| PRJEB82828 | ENA
| S-EPMC5828984 | biostudies-literature
| S-EPMC3653371 | biostudies-literature
| S-EPMC7804374 | biostudies-literature
| S-EPMC5567693 | biostudies-literature
| S-EPMC6116836 | biostudies-literature