Ontology highlight
ABSTRACT:
SUBMITTER: Hockemeyer D
PROVIDER: S-EPMC4142824 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Hockemeyer Dirk D Soldner Frank F Beard Caroline C Gao Qing Q Mitalipova Maisam M DeKelver Russell C RC Katibah George E GE Amora Ranier R Boydston Elizabeth A EA Zeitler Bryan B Meng Xiangdong X Miller Jeffrey C JC Zhang Lei L Rebar Edward J EJ Gregory Philip D PD Urnov Fyodor D FD Jaenisch Rudolf R
Nature biotechnology 20090813 9
Realizing the full potential of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) requires efficient methods for genetic modification. However, techniques to generate cell type-specific lineage reporters, as well as reliable tools to disrupt, repair or overexpress genes by gene targeting, are inefficient at best and thus are not routinely used. Here we report the highly efficient targeting of three genes in human pluripotent cells using zinc-finger nuclease (ZFN)-med ...[more]