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Nanoscale label-free bioprobes to detect intracellular proteins in single living cells.

ABSTRACT: Fluorescent labeling techniques have been widely used in live cell studies; however, the labeling processes can be laborious and challenging for use in non-transfectable cells, and labels can interfere with protein functions. While label-free biosensors have been realized by nanofabrication, a method to track intracellular protein dynamics in real-time, in situ and in living cells has not been found. Here we present the first demonstration of label-free detection of intracellular p53 protein dynamics through a nanoscale surface plasmon-polariton fiber-tip-probe (FTP).

SUBMITTER: Hong W

PROVIDER: S-EPMC4143788 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Nanoscale label-free bioprobes to detect intracellular proteins in single living cells.

Hong Wooyoung W Liang Feng F Schaak Diane D Loncar Marko M Quan Qimin Q

Scientific reports 20140826

Fluorescent labeling techniques have been widely used in live cell studies; however, the labeling processes can be laborious and challenging for use in non-transfectable cells, and labels can interfere with protein functions. While label-free biosensors have been realized by nanofabrication, a method to track intracellular protein dynamics in real-time, in situ and in living cells has not been found. Here we present the first demonstration of label-free detection of intracellular p53 protein dyn ...[more]

PMID: 25154394

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