GluCl a target of indole alkaloid okaramines: a 25 year enigma solved.

ABSTRACT: In 1989, indole alkaloid okaramines isolated from the fermentation products of Penicillium simplicissimum were shown to be insecticidal, yet the mechanism of their toxicity to insects remains unknown. We therefore examined the action of okaramine B on silkworm larval neurons using patch-clamp electrophysiology. Okaramine B induced inward currents which reversed close to the chloride equilibrium potential and were blocked by fipronil. Thus it was tested on the silkworm RDL (resistant-to-dieldrin) ?-aminobutyric-acid-gated chloride channel (GABACl) and a silkworm L-glutamate-gated chloride channel (GluCl) expressed in Xenopus laevis oocytes. Okaramine B activated GluCl, but not RDL. GluCl activation by okaramines correlated with their insecticidal activity, offering a solution to a long-standing enigma concerning their insecticidal actions. Also, unlike ivermectin, okaramine B was inactive at 10 ?M on human ?1?2?2 GABACl and ?1? glycine-gated chloride channels and provides a new lead for the development of safe insect control chemicals.

SUBMITTER: Furutani S 

PROVIDER: S-EPMC4143795 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature