Complex pathways in folding of protein G explored by simulation and experiment.
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ABSTRACT:
SUBMITTER: Lapidus LJ
PROVIDER: S-EPMC4147789 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Publications
Complex pathways in folding of protein G explored by simulation and experiment.
Lapidus Lisa J LJ Acharya Srabasti S Schwantes Christian R CR Wu Ling L Shukla Diwakar D King Michael M DeCamp Stephen J SJ Pande Vijay S VS
Biophysical journal 20140801 4
The B1 domain of protein G has been a classic model system of folding for decades, the subject of numerous experimental and computational studies. Most of the experimental work has focused on whether the protein folds via an intermediate, but the evidence is mostly limited to relatively slow kinetic observations with a few structural probes. In this work we observe folding on the submillisecond timescale with microfluidic mixers using a variety of probes including tryptophan fluorescence, circul ...[more]
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