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124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics.

ABSTRACT: Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes.

SUBMITTER: Wade OK

PROVIDER: S-EPMC6463241 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics.

Wade Orsolya K OK Woehrstein Johannes B JB Nickels Philipp C PC Strauss Sebastian S Stehr Florian F Stein Johannes J Schueder Florian F Strauss Maximilian T MT Ganji Mahipal M Schnitzbauer Joerg J Grabmayr Heinrich H Yin Peng P Schwille Petra P Jungmann Ralf R

Nano letters 20190313 4

Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origa ...[more]

PMID: 30864449

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