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Microbial genomic analysis reveals the essential role of inflammation in bacteria-induced colorectal cancer.

ABSTRACT: Enterobacteria, especially Escherichia coli, are abundant in patients with inflammatory bowel disease or colorectal cancer (CRC). However, it is unclear whether cancer is promoted by inflammation-induced expansion of E. coli and/or changes in expression of specific microbial genes. Here we use longitudinal (2, 12 and 20 weeks) 16S rRNA sequencing of luminal microbiota from ex-germ-free mice to show that inflamed Il10(-/-) mice maintain a higher abundance of Enterobacteriaceae than healthy wild-type mice. Experiments with mono-colonized Il10(-/-) mice reveal that host inflammation is necessary for E. coli cancer-promoting activity. RNA-sequence analysis indicates significant changes in E. coli gene catalogue in Il10(-/-) mice, with changes mostly driven by adaptation to the intestinal environment. Expression of specific genes present in the tumour-promoting E. coli pks island are modulated by inflammation/CRC development. Thus, progression of inflammation in Il10(-/-) mice supports Enterobacteriaceae and alters a small subset of microbial genes important for tumour development.

SUBMITTER: Arthur JC

PROVIDER: S-EPMC4155410 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Microbial genomic analysis reveals the essential role of inflammation in bacteria-induced colorectal cancer.

Arthur Janelle C JC Gharaibeh Raad Z RZ Mühlbauer Marcus M Perez-Chanona Ernesto E Uronis Joshua M JM McCafferty Jonathan J Fodor Anthony A AA Jobin Christian C

Nature communications 20140903

Enterobacteria, especially Escherichia coli, are abundant in patients with inflammatory bowel disease or colorectal cancer (CRC). However, it is unclear whether cancer is promoted by inflammation-induced expansion of E. coli and/or changes in expression of specific microbial genes. Here we use longitudinal (2, 12 and 20 weeks) 16S rRNA sequencing of luminal microbiota from ex-germ-free mice to show that inflamed Il10(-/-) mice maintain a higher abundance of Enterobacteriaceae than healthy wild-t ...[more]

PMID: 25182170

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Microbial genomic analysis reveals the essential role of inflammation in bacteria-induced colorectal cancer.

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Microbial genomic analysis reveals the essential role of inflammation in bacteria-induced colorectal cancer.

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