Inflammation-induced interstitial migration of effector CD4? T cells is dependent on integrin ?V.

ABSTRACT: Leukocytes must traverse inflamed tissues to effectively control local infection. Although motility in dense tissues seems to be integrin independent and based on actomyosin-mediated protrusion and contraction, during inflammation, changes to the extracellular matrix (ECM) may necessitate distinct motility requirements. Indeed, we found that the interstitial motility of T cells was critically dependent on Arg-Gly-Asp (RGD)-binding integrins in the inflamed dermis. Inflammation-induced deposition of fibronectin was functionally linked to higher expression of integrin ?V on effector CD4? T cells. By intravital multiphoton imaging, we found that the motility of CD4? T cells was dependent on ?V expression. Selective blockade or knockdown of ?V arrested T helper type 1 (TH1) cells in the inflamed tissue and attenuated local effector function. Our data demonstrate context-dependent specificity of lymphocyte movement in inflamed tissues that is essential for protective immunity.

SUBMITTER: Overstreet MG 

PROVIDER: S-EPMC4159184 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature