Spherical Aberration-Corrected Metalens for Polarization Multiplexed Imaging.
Ontology highlight
ABSTRACT:
SUBMITTER: Zhou S
PROVIDER: S-EPMC8624245 | biostudies-literature |
REPOSITORIES: biostudies-literature
Similar Datasets
Project description:Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.
| S-EPMC4161287 | biostudies-literature
Project description:We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.
| S-EPMC4180771 | biostudies-literature
Project description:Cryogenic fluorescent light microscopy of flash-frozen cells stands out by artifact-free fixation and very little photobleaching of the fluorophores used. To attain the highest level of resolution, aberration-free immersion objectives with accurately matched immersion media are required, but both do not exist for imaging below the glass-transition temperature of water. Here, we resolve this challenge by combining a cryoimmersion medium, HFE-7200, which matches the refractive index of room-temperature water, with a technological concept in which the body of the objective and the front lens are not in thermal equilibrium. We implemented this concept by replacing the metallic front-lens mount of a standard bioimaging water immersion objective with an insulating ceramic mount heated around its perimeter. In this way, the objective metal housing can be maintained at room temperature, while creating a thermally shielded cold microenvironment around the sample and front lens. To demonstrate the range of potential applications, we show that our method can provide superior contrast in Escherichia coli and yeast cells expressing fluorescent proteins and resolve submicrometer structures in multicolor immunolabeled human bone osteosarcoma epithelial (U2OS) cells at [Formula: see text]C.
| S-EPMC5819432 | biostudies-literature
Project description:Metalenses are instruments that manipulate waves and have exhibited remarkable capabilities to date. However, an important hurdle arises due to the severe hampering of the angular response originating from coma and field curvature aberrations, which result in a loss of focusing ability. Herein, we provide a blueprint by introducing the notion of a wide field-of-hearing (FOH) metalens, designed particularly for capturing and focusing sound with decreased aberrations. Employing an aberration-free planar-thin metalens that leverages perfect acoustic symmetry conversion, we experimentally realize a robust wide FOH capability of approximately 140∘ in angular range. Moreover, our metalens features a relatively short focal length, enabling compact implementation by reducing the aperture-to-hearing plane distance. This is beneficial for space-efficient source-tracking sound sensing. Our strategy can be used across various platforms, potentially including energy harvesting, monitoring, imaging, and communication in auditory, ultrasonic, and submerged environments.
| S-EPMC11001966 | biostudies-literature
Project description:Screw dislocations play an important role in materials' mechanical, electrical and optical properties. However, imaging the atomic displacements in screw dislocations remains challenging. Although advanced electron microscopy techniques have allowed atomic-scale characterization of edge dislocations from the conventional end-on view, for screw dislocations, the atoms are predominantly displaced parallel to the dislocation line, and therefore the screw displacements are parallel to the electron beam and become invisible when viewed end-on. Here we show that screw displacements can be imaged directly with the dislocation lying in a plane transverse to the electron beam by optical sectioning using annular dark field imaging in a scanning transmission electron microscope. Applying this technique to a mixed [a+c] dislocation in GaN allows direct imaging of a screw dissociation with a 1.65-nm dissociation distance, thereby demonstrating a new method for characterizing dislocation core structures.
| S-EPMC4468905 | biostudies-other
Project description:Tomography is an informative imaging modality that is usually implemented by mechanical scanning, owing to the limited depth-of-field (DOF) in conventional systems. However, recent imaging systems are working towards more compact and stable architectures; therefore, developing nonmotion tomography is highly desirable. Here, we propose a metalens-based spectral imaging system with an aplanatic GaN metalens (NA = 0.78), in which large chromatic dispersion is used to access spectral focus tuning and optical zooming in the visible spectrum. After the function of wavelength-switched tomography was confirmed on cascaded samples, this aplanatic metalens is utilized to image microscopic frog egg cells and shows excellent tomographic images with distinct DOF features of the cell membrane and nucleus. Our approach makes good use of the large diffractive dispersion of the metalens and develops a new imaging technique that advances recent informative optical devices.
| S-EPMC6834576 | biostudies-literature
Project description:Metasurfaces in the ultraviolet spectrum have stirred up prevalent research interest due to the increasing demand for ultra-compact and wearable UV optical systems. The limitations of conventional plasmonic metasurfaces operating in transmission mode can be overcome by using a suitable dielectric material. A metalens holds promising wavefront engineering for various applications. Metalenses have developed a breakthrough technology in the advancement of integrated and miniaturized optical devices. However, metalenses utilizing the Pancharatnam-Berry (PB) phase or resonance tuning methodology are restricted to polarization dependence and for various applications, polarization-insensitive metalenses are highly desirable. We propose the design of a high-efficiency dielectric polarization-insensitive UV metalens utilizing cylindrical nanopillars with strong focusing ability, providing full phase delay in a broadband range of Ultraviolet light (270-380 nm). The designed metalens comprises Silicon nitride cylindrical nanopillars with spatially varying radii and offers outstanding polarization-insensitive operation in the broadband UV spectrum. It will significantly promote and boost the integration and miniaturization of the UV photonic devices by overcoming the use of Plasmonics structures that are vulnerable to the absorption and ohmic losses of the metals. The focusing efficiency of the designed metalens is as high as 40%.
| S-EPMC7466348 | biostudies-literature
Project description:The ability to correct the aberrations of the probe-forming lens in the scanning transmission electron microscope provides not only a significant improvement in transverse resolution but in addition brings depth resolution at the nanometer scale. Aberration correction therefore opens up the possibility of 3D imaging by optical sectioning. Here we develop a definition for the depth resolution for scanning transmission electron microscope depth sectioning and present initial results from this method. Objects such as catalytic metal clusters and single atoms on various support materials are imaged in three dimensions with a resolution of several nanometers. Effective focal depth is determined by statistical analysis and the contributing factors are discussed. Finally, current challenges and future capabilities available through new instruments are discussed.
| S-EPMC1413870 | biostudies-literature
Project description:Intracellular symmetry breaking plays a key role in wide range of biological processes, both in single cells and in multicellular organisms. An important class of symmetry-breaking mechanisms relies on the cytoplasm/membrane redistribution of proteins that can autocatalytically promote their own recruitment to the plasma membrane. We present an analytical construction and a comprehensive parametric analysis of stable localized patterns in a reaction-diffusion model of such a mechanism in a spherical cell. The constructed patterns take the form of high-concentration patches localized into spherical caps, similar to the patterns observed in the studies of symmetry breaking in single cells and early embryos.
| S-EPMC6035301 | biostudies-literature
Project description:PURPOSE:To investigate the distribution of corneal spherical aberration (SA) in Tanzanian people of African descent, and to examine the correlation between corneal SA and ocular parameters. DESIGN:Cross-sectional population-based study. METHODS:Residents aged 40 years and older in three villages in the Mkuranga district in Tanzania were enlisted as study participants. Corneal higher-order aberrations (HOAs) for the right eye were measured with a wavefront analyzer (KR-1W, Topcon) and calculated for the central 6.0-mm zone. Corneal curvature radius (CR), corneal astigmatism, and axial length (AL) were also measured and their correlation with corneal SA was assessed. RESULTS:The right eyes of 657 participants (336 male, 321 female) were analyzed. The mean age of the subjects was 57.2 ± 10.3 years (mean ± SD). The mean corneal SA (Zernike spherical aberration coefficient C40) was 0.188 ± 0.095 μm (-0.242 to 0.613). The SAs in about three-quarters of all subjects were between 0.10 and 0.30 μm. The root mean squares of total corneal HOAs and the third- and fourth-order aberrations were 0.629 ± 0.250 μm, 0.539 ± 0.236 μm, and 0.269 ± 0.110 μm, respectively. Corneal SA showed weak significant correlations with CR (Spearman's rank correlation coefficient, r = -0.177, p < 0.001), corneal astigmatism (r = -0.142, p < 0.001), AL (r = -0.168, p < 0.001), and age (r = -0.085, p < 0.05). CONCLUSIONS:This finding may be beneficial for selecting aspheric intraocular lens in this population.
| S-EPMC6742233 | biostudies-other