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Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes.


ABSTRACT: A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells.

SUBMITTER: Lee YJ 

PROVIDER: S-EPMC4184966 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes.

Lee Yan-Jiun YJ   Kurra Yadagiri Y   Yang Yanyan Y   Torres-Kolbus Jessica J   Deiters Alexander A   Liu Wenshe R WR  

Chemical communications (Cambridge, England) 20141101 86


A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells. ...[more]

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