Methylation by NSun2 represses the levels and function of microRNA 125b.
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ABSTRACT:
SUBMITTER: Yuan S
PROVIDER: S-EPMC4187725 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Publications
Methylation by NSun2 represses the levels and function of microRNA 125b.
Yuan Shuai S Tang Hao H Xing Junyue J Fan Xiuqin X Cai Xiaoyu X Li Qiu Q Han Pei P Luo Yuhong Y Zhang Zhuojun Z Jiang Bin B Dou Yali Y Gorospe Myriam M Wang Wengong W
Molecular and cellular biology 20140721 19
Methylation is a prevalent posttranscriptional modification of RNAs. However, whether mammalian microRNAs are methylated is unknown. Here, we show that the tRNA methyltransferase NSun2 methylates primary (pri-miR-125b), precursor (pre-miR-125b), and mature microRNA 125b (miR-125b) in vitro and in vivo. Methylation by NSun2 inhibits the processing of pri-miR-125b2 into pre-miR-125b2, decreases the cleavage of pre-miR-125b2 into miR-125, and attenuates the recruitment of RISC by miR-125, thereby r ...[more]
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