Crystallization and preliminary crystallographic analysis of poly(3-hydroxybutyrate) depolymerase from Bacillus thuringiensis.

ABSTRACT: Poly[(R)-3-hydroxybutyrate] (PHB) is a microbial biopolymer that has been commercialized as biodegradable plastics. The key enzyme for the degradation is PHB depolymerase (PhaZ). A new intracellular PhaZ from Bacillus thuringiensis (BtPhaZ) has been screened for potential applications in polymer biodegradation. Recombinant BtPhaZ was crystallized using 25% polyethylene glycol 3350, 0.2?M ammonium acetate, 0.1?M bis-tris pH 6.5 at 288?K. The crystals belonged to space group P1, with unit-cell parameters a = 42.97, b = 83.23, c = 85.50?Å, ? = 73.45, ? = 82.83, ? = 83.49°. An X-ray diffraction data set was collected to 1.42?Å resolution with an Rmerge of 6.4%. Unexpectedly, a molecular-replacement solution was obtained using the crystal structure of Streptomyces lividans chloroperoxidase as a template, which shares 24% sequence identity to BtPhaZ. This is the first crystal structure of an intracellular poly(3-hydroxybutyrate) depolymerase.

SUBMITTER: Wang YL 

PROVIDER: S-EPMC4188094 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature