Ontology highlight
ABSTRACT:
SUBMITTER: Lu D
PROVIDER: S-EPMC4195823 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Lu Dan D Hsiao Jennifer Y JY Davey Norman E NE Van Voorhis Vanessa A VA Foster Scott A SA Tang Chao C Morgan David O DO
The Journal of cell biology 20141006 1
The ubiquitin protein ligase anaphase-promoting complex or cyclosome (APC/C) controls mitosis by promoting ordered degradation of securin, cyclins, and other proteins. The mechanisms underlying the timing of APC/C substrate degradation are poorly understood. We explored these mechanisms using quantitative fluorescence microscopy of GFP-tagged APC/C(Cdc20) substrates in living budding yeast cells. Degradation of the S cyclin, Clb5, begins early in mitosis, followed 6 min later by the degradation ...[more]